Part:BBa_K733006

ydjM+Bmp2

This is mature Bone Morphogenetic Protein 2 (BMP2) with a singal peptide YdjM. The design is the same as BBa_K733005 but with another signal peptide YdjM. It is also a signal peptide in Bacillus subtilis. For the description of mature BMP2, you can refer to BBa_K733005. For signal peptide YdjM, it has a more accurate cleaving site which ideally cuts the BMP2 from the signal peptide (Tjalsma et al, 2000), compare with the signal peptide YbdN in another construct.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Beck, S. E., Jung, B. H., Fiorino, A., Gomez, J., Del Rosario, E., Cabrera, B. L., Huang, S. C., Chow, J. Y. C., & Carethers J.M. (2006). Bone morphogenetic protein signaling and growth suppression in colon cancer. The American Journal of Physiology-Gastrointestinal and Liver Physiology, 291(1), G135-G145.

Tjalsma, H., Bolhuis, A., Jongbloed, J. D. H., Bron, S., & Dijl, J. M. V. (2000). Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome. Microbiology and Molecular Biology Reviews, 64(3), 515-547.